全國服務咨詢熱線:
15921799099
15921799099
在細胞培養和3D組織工程研究中，膠原蛋白作為細胞外基質的主要成分，是細胞附著、增殖和分化的重要基礎。然而，傳統膠原蛋白產品往往存在純度低、批次間差異大等問題，影響實驗結果的穩定性和重復性。Advanced BioMatrix公司的PureCol® 牛膠原蛋白憑借其高純度、高一致性和多功能性，為這些難題提供了理想的解決方案。作為Advanced BioMatrix公司的中國授權代理，上海起發強烈推薦其PureCol® 牛膠原蛋白產品。
突破膠原蛋白應用困境
純度低、批次間差異大
PureCol®的高純度（>99.9%）和嚴格的質量控制確保了實驗結果的穩定性和重復性。
操作復雜、使用不便
提供即用型溶液，無需額外處理，簡化了實驗流程。
應用場景有限
PureCol®適用于多種細胞培養和3D組織工程應用，為研究提供了廣泛的適用性。
advancedbiomatrix 5005產品優勢盡顯
• 超高純度與天然性
PureCol®膠原蛋白是所有膠原蛋白產品中純度較高的標準（膠原蛋白含量>99.9%），幾乎都由I型膠原蛋白組成。
產品來源于美國受控封閉牛群，確保原料的純凈和安全性。
• 高一致性和可靠性
PureCol®經過嚴格的生產工藝，確保批次間高度一致，被超過1000篇文獻引用，品質均一。
每批產品均附帶分析證書，提供詳細的濃度和質量信息。
• 多功能與廣泛應用
PureCol®適用于多種應用場景，包括組織工程、創傷修復、3D細胞培養、表面涂層等。
提供即用型溶液（3 mg/mL，pH 2），可直接用于細胞培養或作為固體凝膠。
• 用戶友好與即用性
產品經過無菌過濾，以100 mL包裝提供，便于使用和儲存。
提供多種規格（100 mL、1000 mL），滿足不同實驗需求。
訂購信息
PureCol® Bovine Collagen是一種高純度（>99.9%）的I型膠原蛋白溶液，適用于二維和三維細胞培養、表面涂層或作為固體凝膠使用。該產品從美國受控封閉牛群的牛皮中提取，確保原料的純凈和安全性。
品名：PureCol® Bovine Collagen, 3 mg/mL, 100 mL
貨號：5005-100ML
規格：100 mL
濃度：3 mg/mL
儲存溫度：2-10°C
有效期：詳見產品包裝與隨貨發出的COA。
使用方法
1.表面涂層：推薦使用濃度：3 mg/mL。
涂層步驟：將培養皿或支架浸入PureCol®溶液中，室溫下孵育1小時，然后用PBS洗滌。
2.3D細胞培養：將細胞懸液與PureCol®混合，調整至中性pH后進行凝膠化。
凝膠化時間：<40分鐘。
3.凝膠形成：調整pH至中性后，37°C孵育以促進凝膠化。
advancedbiomatrix 5005在3D組織工程中的具體應用
1. 3D打印組織工程支架
牛膠原蛋白被廣泛用于3D打印支架的制備，這些支架可用于修復和再生多種組織，如皮膚、骨骼、軟骨和血管。例如，研究者通過將I型牛膠原蛋白制成纖維狀膠體凝膠，結合3D打印技術制造出具有工程化孔隙結構的支架。這些支架經過化學交聯和凍干后處理，表現出良好的熱穩定性和細胞黏附性。
2. 定制化骨組織工程
牛膠原蛋白還可與其他材料結合，用于定制化骨組織工程。例如，研究者將牛膠原蛋白與β-磷酸三鈣（β-TCP）結合，通過3D打印技術制備出具有多級孔結構的骨支架。這種支架不僅與天然骨組織的力學性能相似，還表現出良好的細胞黏附性和成骨活性。
3. 軟組織修復
牛膠原蛋白在軟組織修復中也有廣泛應用。例如，研究者將牛膠原蛋白與納米纖維素、羧甲基纖維素等材料結合，制備出具有增強機械性能和生物相容性的混合水凝膠。這些水凝膠可用于3D打印，制造出適合軟組織修復的支架。
4. 藥物釋放與組織再生
牛膠原蛋白還可用于制備具有藥物釋放功能的支架。例如，研究者將牛膠原蛋白與聚乳酸結合，制備出能夠控制藥物釋放的納米纖維支架。這種支架不僅能夠促進組織再生，還能有效釋放藥物，抑制感染。
5. 血管化組織工程
牛膠原蛋白在血管化組織工程中也表現出色。例如，研究者利用牛膠原蛋白水凝膠支架，成功促進了血管內皮細胞的生長和新生血管的形成。這種支架在體內植入后，能夠逐漸降解，同時支持組織的再生。
參考文獻引用
【1】《PureCol EZ 凝膠，牛膠原 DMEM/F-12 溶液的制備方法》：介紹了 Advanced BioMatrix PureCol EZ 凝膠的成分、特性及三維凝膠的制備方法等，提到該產品是高度純化的無氨基膠原蛋白，約 97% 為 I 型膠原蛋白，其余為 III 型膠原蛋白，能為細胞培養提供長效的 L - 谷氨酰胺來源，可用于研究細胞外基質機械性能對細胞的影響等。
【2】《PureCol-S，膠原蛋白標準品的應用與特性分析》：對 Advanced BioMatrix 的 PureCol®-S 膠原蛋白溶液進行了分析，指出其濃度約為 3mg/mL，pH 值為 2，純度≥99.9%，約 97% 為 I 型膠原蛋白，其余為 III 型膠原蛋白，適用于作為膠原蛋白分析標準，還介紹了其來源、制備工藝及儲存條件等。
【3】《PureCol 水凝膠和間隙流》：提到研究人員使用 PureCol® I 型膠原蛋白（3 毫克 / 毫升，型號 5005-100ml）水凝膠來探究緩慢的間質液流動如何激活 TGF-β 信號傳導，得出緩慢的間質液流動足以誘發纖維化等結論，展示了 PureCol® 在相關細胞信號傳導研究中的應用。
Advanced BioMatrix 是一家位于美國圣地亞哥的生物技術公司，專注于組織培養、細胞檢測、生物打印和細胞增殖的三維（3D）應用。公司致力于通過創新的3D矩陣產品提供先進的技術，將科學研究提升到新的發現水平。
advancedbiomatrix熱賣產品
| 貨號 | 品名 | 規格 | 品牌 |
| 5005-100ML | PureCol®, Bovine Collagen, 3 mg/mL, 100 mL | 100ml | advancedbiomatrix |
| 5153-1KIT | Rat Tail Collagen Solution, Acid Soluble with Neutralization Solution, 100 mg | 1KIT | advancedbiomatrix |
| 5007-20ml | Type I Collagen Solution, 3 mg/ml (Human) | 20ml | advancedbiomatrix |
| 5022-5MG | Human Collagen, Type IV | 5MG | advancedbiomatrix |
| 5133-20ML | FibriCol® | 20ml | advancedbiomatrix |
| 5074-35ML | PureCol® EZ Gel, Bovine Collagen, DMEM/F-12 Solution | 35ML | advancedbiomatrix |
| 5248-1KIT | Ruthenium, Visible Light Photocrosslinking Kit | 1KIT | advancedbiomatrix |
| 5015 | PureCol®-S Type I Collagen Standard | 20ml | advancedbiomatrix |
| 5144-5EA | CytoSoft® 6-well Plate, Elastic Modulus 32 kPa | 5EA | advancedbiomatrix |
| 5190-7EA | CytoSoft® Discovery Kit | 7EA | advancedbiomatrix |
| 5141-5EA | CytoSoft® 6-well Plate, Elastic Modulus 2kPa | 5EA | advancedbiomatrix |
| 5021-10MG | Human Collagen Solution, Type III, 10 mg | 10MG | advancedbiomatrix |
| 5050-1MG | Fibronectin, Human, 1.0 mg | 1MG | advancedbiomatrix |
| 5142-5EA | CytoSoft® 6-well Plate, Elastic Modulus 8 kPa | 5EA | advancedbiomatrix |
| 5010-50ML | Type I Collagen Solution, 6 mg/ml (Bovine) | 50mL | advancedbiomatrix |
| 5026 | TeloCol®-3 Type I Collagen Solution, 3 mg/ml (Bovine) | 1kit | advancedbiomatrix |
| 5143-5EA | CytoSoft® 6-well Plate, Elastic Modulus 16 kPa | 5EA | advancedbiomatrix |
| 5196-0.5MG | Bovine Collagen Solution, Type II, 0.5 mg/mL, 1mL mg/ml | 0.5MG | advancedbiomatrix |
| 5226-20ML | TeloCol®-10 | 20ml | advancedbiomatrix |
| 5145-5EA | CytoSoft® 6-well Plate, Elastic Modulus 64 kPa | 5EA | advancedbiomatrix |
| 5165-5EA | CytoSoft® 6-well Plate, Elastic Modulus 0.2 kPa | 5EA | advancedbiomatrix |
| 5198-100MG | PhotoCol® Methacrylated Type I Collagen (Collagen Only) | 100MG | advancedbiomatrix |
| GS310 | HyStem® Thiol-Modified Hyaluronan Hydrogel Kit | kit | advancedbiomatrix |
| GS311F | HyStem® Thiol-Modified Hyaluronic Acid Kit 7.5 mL | 7.5ml | advancedbiomatrix |
| 5005-1000ML | PureCol®, Bovine Collagen, 3 mg/mL, 1000 mL | 1000ML | advancedbiomatrix |
| 5008 | VitroCol® Lyophilized, 15 mg | 15 mg | advancedbiomatrix |
| 5162-1GM | Insoluble Collagen Type I Collagen, Insoluble Fibrous Powder (Bovine) | 1GM | advancedbiomatrix |
| 5201-1KIT | PhotoCol®-IRG | 1kit | advancedbiomatrix |
| 5056-20ML | Rat Tail Collagen Solution, Acid Soluble, 4 mg/ml | 20ML | advancedbiomatrix |
| 5154-20ML | Silk Fibroin Solution, 50 mg/ml | 20ML | advancedbiomatrix |
| 5225-1KIT | TeloCol®-6, Solution, Acid Soluble, 6 mg/ml + Neutralization Solution | 1KIT | advancedbiomatrix |
| GS312 | HyStem-C Thiol-Modified Hyaluronic Acid + Gelatin Kit 2.5 mL Trial Kit | kit | advancedbiomatrix |
| IKD119261001 | Rat Tail Collagen, Type I, Solution, 10 mg/ml, 10 mL | 10ml | advancedbiomatrix |
| 5007-100ML | VitroCol®, Human Collagen, Solution, 3 mg/mL | 100ML | advancedbiomatrix |
| 5016-5MG | Human Collagen, Type IV, Powder, Non-sterile, 5 mg | 5MG | advancedbiomatrix |
| 5052-1MG | Tropoelastin, Human, Recombinant, Lyophilized, 1 mg | 1MG | advancedbiomatrix |
| 5079-50ml | Acetic Acid Solution, 20 mM, 50 mL | 50ml | advancedbiomatrix |
| 5162 | Type I Collagen, Insoluble Fibrous Powder (Bovine) | 1g | advancedbiomatrix |
| 5200-5gm | irgacure 5929 photoinitiator 5 grams non-sterile | 5gm | advancedbiomatrix |
| 5257-1EA | CytoSoft® Imaging 96-well Plate, Elastic Modulus 2 kPa | 1EA | advancedbiomatrix |
| 5406-2EA | iMatrix-111, Recombinant Laminin 111, 0.5 mg/ml, 2 x 0.35 mL | 2EA | advancedbiomatrix |
| GS231F-5EA | Gelin-S® Thiol-Modified Gelatin (5 units x 1 mL) | 5EA | advancedbiomatrix |
| GS313F | HyStem®-C Thiol-Modified Hyaluronic Acid + Gelatin Kit 7.5 mL | 7.5ml | advancedbiomatrix |
| GS315F | HyStem®-HP Thiol-Modified Hyaluronic Acid/Heparin Kit 7.5 mL | 7.5ml | advancedbiomatrix |
| 5006-15MG | PureCol®, Bovine Collagen, Lyophilized, 15 mg | 15MG | advancedbiomatrix |
| 5140-5EA | CytoSoft® 6-well Plate, Elastic Modulus 0.5 kPa | 5EA | advancedbiomatrix |
| 5183-1EA | CytoSoft® Imaging 24-well Plate, Elastic Modulus 0.2 kPa | 1EA | advancedbiomatrix |
| 5212-100MG | Methacrylated 100MGHyaluronic Acid (HAMA) | 100MG | advancedbiomatrix |
| 5255-1EA | CytoSoft® Imaging 96-well Plate, Elastic Modulus 0.2 kPa | 1EA | advancedbiomatrix |
| 5256-1EA | CytoSoft® Imaging 96-well Plate, Elastic Modulus 0.5 kPa | 1EA | advancedbiomatrix |
| 5261-1EA | CytoSoft® Imaging 96-well Plate, Elastic Modulus 64 kPa | 1EA | advancedbiomatrix |
| 5270-1KIT | PhotoCol®-LAP | 1kit | advancedbiomatrix |
| GS1005F | HyStem®-C Thiol-Modified Hyaluronic Acid + Gelatin 12.5 mL Kit (Experienced Users Only) | 12.5ml | advancedbiomatrix |
| GS222-5EA | Glycosil Thiol-Modified hyaluronic acid (5 units x 1 mL) | 5unitsx1mL | advancedbiomatrix |
| GS222F-5EA | Glycosil® Thiol-Modified hyaluronic acid, 5 x 1 mL | 5ea | advancedbiomatrix |
| GS3007F-10EA | Extralink® PEGDA, 10 x 0.5 mL | 10ea | advancedbiomatrix |
| gs311 | HyStem Hydrogel Kit, 7.5 mL | 7.5ml | advancedbiomatrix |
| 5019-10MG | Human Collagen, Type III, Powder, Non-sterile, 10 mg | 10MG | advancedbiomatrix |
| 5051-0.1MG | Vitronectin, Human, 0.1 mg | 0.1MG | advancedbiomatrix |
| 5073-5EA | 6-well Plates, PureCol® Collagen Coated | 5ea | advancedbiomatrix |
| 5080-5MG | Fibronectin, Human, Lyophilized 5.0 mg | 5MG | advancedbiomatrix |
| 5133-100ML | FibriCol®, Bovine Atelocollagen Solution, 10 mg/ml, 100 mL | 100ML | advancedbiomatrix |
| 5135-25EA | SpongeCol®, Collagen Sponges, Columnar Pore Architecture, 4 mm Diameter Discs | 25ea | advancedbiomatrix |
| 5170-1EA | ECM Select® Array Kit Ultra-36 | 1EA | advancedbiomatrix |
| 5185-1EA | CytoSoft® Imaging 24-well Plate, Elastic Modulus 2 kPa | 1EA | advancedbiomatrix |
| 5186-1EA | CytoSoft® Imaging 24-well Plate, Elastic Modulus 8 kPa | 1EA | advancedbiomatrix |
| 5188-1EA | CytoSoft® Imaging 24-well Plate, Elastic Modulus 32 kPa | 1EA | advancedbiomatrix |
| 5189-1EA | CytoSoft® Imaging 24-well Plate, Elastic Modulus 64 kPa | 1EA | advancedbiomatrix |
| 5220-1KIT | PhotoHA®-IRG Methacrylated Hyaluronic Acid with Irgacure Kit | 1kit | advancedbiomatrix |
| 5258-1EA | CytoSoft® Imaging 96-well Plate, Elastic Modulus 8 kPa | 1EA | advancedbiomatrix |
| 5259-1EA | CytoSoft® Imaging 96-well Plate, Elastic Modulus 16 kPa | 1EA | advancedbiomatrix |
| 5265-60UG | Biotin Conjugate Collagen Hybridizing Peptide (Biotin) | 60UG | advancedbiomatrix |
| GS1004F | HyStem® Thiol-Modified Hyaluronic Acid 12.5 mL Kit (Experienced Users Only) | 12.5ml | advancedbiomatrix |
| GS1006F | HyStem®-HP Thiol-Modified Hyaluronic Acid/Heparin 12.5 mL Kit (Experienced Users Only) | 12.5ml | advancedbiomatrix |
| GS217F-5EA | Heprasil® Thiol-Modified hyaluronic Acid/Heparin, 5 x 1 mL | 5ea | advancedbiomatrix |
| GS220F-2EA | Glycosil® Thiol-Modified hyaluronic acid, 2 x 5 mL | 2ea | advancedbiomatrix |
| GS3006F-5EA | Extralink® PEGDA, 5 x 2.5 mL | 5EA | advancedbiomatrix |
| GS3008F-5EA | Extralink®-Lite PEGDA, 5 x 2.5 mL | 5ea | advancedbiomatrix |
| GS3009-10EA | Extralink®-Lite PEGDA, 10 x 0.5 mL | 10ea | advancedbiomatrix |
| GS314 | HyStem-HP Thiol-Modified Hyaluronic Acid/Heparin Kit 2. mL | kit | advancedbiomatrix |
| 10-523-001 | EBV Nuclear Antigen-1 (EBNA-1) Recombinant Protein | 200ug | advancedbiomatrix |
| 5010-D | Nutragen® Bovine Collagen | each | advancedbiomatrix |
| 5020-5MG | PEPTITE-2000®, RGD Peptide, 5 mg | 5MG | advancedbiomatrix |
| 5029-10EA | T-25 Flasks, PureCol® Collagen Coated | 10EA | advancedbiomatrix |
| 5049-50ML | Poly-D-Lysine, Solution, 0.1 mg/mL | 50ML | advancedbiomatrix |
| 5058-50ML | Poly-L-Ornithine, Solution, 0.1 mg/mL, 50 mL | 50ML | advancedbiomatrix |
| 5135-5EA | SpongeCol®, Collagen Sponges, Columnar Pore Architecture, 21 mm Diameter Discs | 5ea | advancedbiomatrix |
| 5138-10GM | SphereCol®, Human Type I Collagen Coated Beads, Sterile, 10 grams | 10GM | advancedbiomatrix |
| 5173-100MG | Poly-L-Lysine, Non-Sterile Powder, 100 mg | 100MG | advancedbiomatrix |
| 5174-100MG | Poly-D-Lysine, Non-Sterile Powder, 100 mg | 100MG | advancedbiomatrix |
| 5174-5MG | Poly-D-Lysine, Powder, 5 mg | 5MG | advancedbiomatrix |
| 5184-1EA | CytoSoft® Imaging 24-well Plate, Elastic Modulus 0.5 kPa | 1EA | advancedbiomatrix |
| 5187-1EA | CytoSoft® Imaging 24-well Plate, Elastic Modulus 16 kPa | 1EA | advancedbiomatrix |
| 5260-1EA | CytoSoft® Imaging 96-well Plate, Elastic Modulus 32 kPa | 1EA | advancedbiomatrix |
| 5264-60UG | 5-FAM Conjugate Collagen Hybridizing Peptide (Green) | 60UG | advancedbiomatrix |
| 5267-5ML | Lifeink® 240 Collagen Bioink, Acidic pH, 35 mg/ml, 5 mL | 5ML | advancedbiomatrix |
| 5269-500mg | LAP | 500mg | advancedbiomatrix |
| 5276-60UG | CY3 Conjugate Collagen Hybridizing Peptide (Red) | 60UG | advancedbiomatrix |
| 5278-5ML | Lifeink® 200 Collagen Bioink, pH Neutral, 35 mg/ml | 5ML | advancedbiomatrix |
電話
微信
當前位置：
地址：上海浦東川沙鎮川沙路6619號上海起發實驗試劑有限公司
郵箱：xs1@78bio.com
傳真：021-50724961