Glen Research/Complement Technology/Abbexa廠家答疑與實驗優化指南

●問題1

貨號：10-5000-02M

品名：Universal-CE Phosphoramidite -（10-5000）

品牌：Glen Research

原文：

Q:“10-5000-02M"?When coupling this product with hydroxyl groups, do we need to follow the same procedure as in chemical synthesis, i.e., first add it to the mixed solution of it and ETT, then cap and oxidize? Or can we directly immerse the solid-phase carrier with hydroxyl groups in water for 12 hours?

A:Yes, our product Universal-CE Phosphoramidite - (10-5000) should follow the same procedures as other the phosphoramidite reagents (Activation & coupling, capping, oxidation). Like other any phosphoramidites water should not be involved in the coupling of these reagents. Phosphoramidites are highly sensitive to water because it causes rapid hydrolysis destroying its ability to couple during oligonucleotide synthesis.

譯文：

問：針對產品“10-5000-02M"，將其與羥基偶聯時，是否需要遵循化學合成的常規流程，即先將其加入該產物與ETT的混合溶液中，隨后進行封端和氧化？還是說我們可以直接將帶有羥基的固相載體浸入水中放置12小時？

答：是的，我們的產品 Universal-CE 亞磷酰胺 (10-5000) 應遵循與其他亞磷酰胺試劑相同的操作流程（活化與偶聯、封端、氧化）。與其他所有亞磷酰胺一樣，此類試劑的偶聯過程嚴禁接觸水。亞磷酰胺對水極其敏感，遇水會發生快速水解，從而喪失其在寡核苷酸合成中的偶聯能力。

●問題2

貨號：NHS

品名：NHS試劑

品牌：Complement Technology

原文：

Q:Is this Human Serum derived from multiple donors or a single donor？Also, do you have any recent literature published using this product, or any protocols for its use?

A:No, you would have to look up paper, etc.  There should be some in the scientific literature databases.

譯文：

問：這種人血清是來源于多名供體，還是單一供體？另外，請問是否有近期發表的、使用了該產品的文獻，或者相關的使用方案？

答：沒有。你需要自行查閱論文等資料，科學文獻數據庫中應該能找到一些相關信息。

●問題3

貨號：A114

品名：C3b 1.0 mg/ml

品牌：Complement Technology

原文：

Q:What is the approximate percentage of activity for this product

A:There is no activiy.  It is C3b (fragment of C3).

譯文：

問：請問該產品的活性百分比約為多少？

答：無活性。其為 C3b（補體成分 C3 的裂解片段）。

●問題4

貨號：Rb114/R114/M114

品名：Rabbit C3b/Rat C3b/Mouse C3b

品牌：Complement Technology

原文：

Q:What is the approximate percentage of activity for this product

A:C3b is a fragment of C3.  It has no activity.

For C3b there is no functional assay in the CoA. However, we do an in-house functional assay wherein using SDS-PAGE analysis we determine the cleavage of C3b to iC3b by FI in the presence of cofactor FH.

We do evaluate all our C3b preparations in an in-house functional assay but we do not report it. We analyze Factor I cleavage of C3b to iC3b in the presence of the cofactor Factor H by SDS-PAGE under reduced conditions. C3b under reduced conditions shows two bands of about 101,000 daltons for the alpha prime chain (α’-chain) and 75,000 daltons for the beta chain. In the presence of cofactor Factor H, Factor I cleaves the alpha prime chain of C3b (101,000 daltons) yielding two fragments (63,000 and 39,000 Da). Thus, when C3b is converted to iC3b we see the disappearance of the alpha prime chain and the appearance of two bands @ 63,000 and 39,000 Da in addition to the beta chain @ 75,000 daltons. Functional analysis of C3b indicates that our C3b preparations are > 90% active.

譯文：

問：該產品的大致活性百分比是多少？

答：C3b 是 C3 的一個片段。它本身沒有（溶血）活性。

對于 C3b，在產品分析證書（CoA）中沒有功能性檢測。但是，我們會進行一項內部功能性檢測，即通過 SDS-PAGE 分析，測定在輔因子 H（FH）存在下，因子 I（FI）將 C3b 裂解為 iC3b 的情況。

我們會通過這項內部功能性檢測評估所有 C3b 制劑，但不將其報告在證書中。我們通過還原條件下的 SDS-PAGE，分析在輔因子 H 存在時因子 I 對 C3b 的裂解情況。在還原條件下，C3b 顯示兩條條帶：約 101,000 道爾頓的 α' 鏈（α'-鏈）和 75,000 道爾頓的 β 鏈。在輔因子 H 存在的情況下，因子 I 會切割 C3b 的 α' 鏈（101,000 道爾頓），產生兩個片段（63,000 和 39,000 道爾頓）。因此，當 C3b 轉化為 iC3b 時，我們會觀察到 α' 鏈的消失，并出現位于 63,000 和 39,000 道爾頓處的兩條新條帶，此外還有位于 75,000 道爾頓處的 β 鏈。

對 C3b 的功能分析表明，我們的 C3b 制劑活性 > 90%。

●問題5

貨號：abx670024

品名：Human Complement C3b (C3b) Protein

品牌：Abbexa

原文：

Q:What is the approximate percentage of activity for this product?

A:The activity of this protein has not been tested.

譯文：

問：請問該產品的活性百分比約為多少？

答：該蛋白的活性尚未經檢測。

更多產品信息，請聯系中國區域代理商：上海起發實驗試劑有限公司

（注：本文內容基于品牌公開資料及行業常規信息整理，具體以品牌信息文檔為準。）